Inactive doses of oxathiine derivative on long-term effects of the organism

Introduction. The study of the effects of the long-term effect of the active substances of pesticides on the body of warm-blooded animals is an obligatory stage of their comprehensive toxicological and hygienic assessment.

The aim of the study was to establish the levels of inactive doses of the effects of the long-term (NOEL) action of a compound of the oxatiine class.

Materials and methods. In accordance with the planned purpose of the study in the biological testing laboratory of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing еxperiments were conducted to establish the parameters of acute oral toxicity, the presence of possible teratogenic and embryotoxic effects, and the effect on the reproductive function of a technical product of the oxatiin class (TPO) on the body of warm-blooded (rats). Previously, studies in this volume have not been conducted.

Results. LD₅₀ of the studied compound with a single oral administration to male rats is more than 4500 mg/kg of body weight. When studying the embryotoxic effect, the inactive dose for the maternal organism and offspring is set at the level of — 1/52 LD₅₀; the inactive dose for the teratogenic effect for the maternal organism is 1/52 LD₅₀, offspring — 1/27 LD₅₀. As a result of studying the effect of the oxatiine derivative on the indicators of reproductive function, the inactive dose for maternal, paternal organisms, and offspring, is 1/313 LD₅₀.

Limitations. In our study to establish the levels of inactive doses of the long-term effects of toxicity of a technical product derived from oxatiines, there are no data on the results of the mutagenic effect of the studied compound on the body of warm-blooded (rats, mice).

Conclusion. It has been established that according to the “Hygienic classification of pesticides and agrochemicals according to the degree of danger” (Methodological Recommendations of MP No. 1.2.0235-21* dated 02/15/2022), the studied chemical product belongs to the 4th hazard class for acute oral toxicity (LD₅₀) and teratogenic effect (low-hazard compound; for embryotoxic effect and reproductive toxicity to the 3rd hazard class (moderately dangerous compound).

Compliance with ethical standards. The conduct of the study was approved by the commission of the Ethics Committee of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing, Mytishchi, 141014, Russian Federation (Protocol of the meeting No. 9 dated 15.09.2022).

Contribution:
Rakitskii V.N., Kuzmin S.V. — the concept and design of the study, editing;
Mukhina E.A. — conducting an experiment, collection and statistical processing of primary material;
Epishina T.M. — conducting an experiment, collection and statistical processing of primary material, writing text, compiling a list of references;
Chkhvirkia E.G. — writing text, compilation of the list of references.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: September 8, 2022 / Accepted: March 24, 2023 / Published: April 20, 2023

* Methodological recommendations. MP No. 1.2.0235–21 of 03/15/2021 Hygienic classification of pesticides and agrochemicals according to the degree of danger. Moscow: 2021; 13 p.