New Tiaoxin Recipe Alleviates Energy Metabolism Disorders in an APPswe/PS1DE9 Mouse Model of Alzheimers Disease
- Авторлар: Hu Y.1, Xing S.2, Huang Y.2, Chen C.2, Shen D.2, Chen J.2
-
Мекемелер:
- , Shanghai University of Traditional Chinese Medicine
- , Shanghai Geriatric Institute of Chinese Medicine
- Шығарылым: Том 27, № 4 (2024)
- Беттер: 621-631
- Бөлім: Chemistry
- URL: https://rjraap.com/1386-2073/article/view/644768
- DOI: https://doi.org/10.2174/1386207326666230428112358
- ID: 644768
Дәйексөз келтіру
Толық мәтін
Аннотация
Background:Alzheimer's disease (AD) is a typical neurodegenerative disease with a complex etiology. Until now, there has been no effective treatment available for AD; however, improving energy dysmetabolism, the key pathological event in the early stage of AD, can effectively delay the progression of AD.
Objective:This paper aims to investigate the therapeutic effect and potential mechanism of the new Tiaoxin recipe on early AD.
Methods:APP/PS1 mice were divided into a model group, a new Tiaoxin recipe group, and a donepezil group, and C57/BL mice were used for the control group. Mouse cognitive and learning abilities were tested using the Morris water maze test and a new object-recognition experiment. The 42 amino acid form of amyloid β peptide (Aβ142) content was detected by enzyme-linked immunosorbent assay, the senile plaque area was detected by thioflavin S staining, and the senescence- associated β-galactosidase (SA-β-gal)positive area was detected by chemical staining. Also, the adenosine triphosphate (ATP), nicotinamide adenine dinucleotide (NAD+), and nicotinamide adenine dinucleotide hydride (NADH) contents were detected using a biochemical method, and the cluster of differentiation 38 (CD38) and silent matingtype information regulation 2 homolog 3 (SIRT3) protein expression levels were detected by immunofluorescence and Western blot analysis.
Results:Compared with those of the control group, the learning and memory abilities of the model group were impaired; the senile plaque deposition, Aβ142 content, and SA-βgalpositive staining area were increased; the ATP concentration, NAD+ concentration, and NAD+/NADH ratio were decreased; the CD38 protein expression level was increased; and the SIRT3 protein expression level was decreased. Following intervention with the new Tiaoxin recipe, the learning and memory abilities were improved; the senile plaque deposition, Aβ142 content, and SA-βgalpositive area were reduced; the ATP concentration, NAD+ concentration, and NAD+/NADH ratio were increased; CD38 protein expression was decreased, and SIRT3 protein expression was increased.
Conclusion:This study shows that the new Tiaoxin Recipe can improve cognitive ability and reduce the Aβ1-42 content and senile plaque deposition in APP/PS1 mice, which may occur through the downregulation of CD38 protein expression, upregulation of SIRT3 protein expression, restoration of the NAD+ level, promotion of ATP synthesis, mitigation of energy metabolism disorders.
Негізгі сөздер
Авторлар туралы
Yiran Hu
, Shanghai University of Traditional Chinese Medicine
Email: info@benthamscience.net
Sanli Xing
, Shanghai Geriatric Institute of Chinese Medicine
Хат алмасуға жауапты Автор.
Email: info@benthamscience.net
Yan Huang
, Shanghai Geriatric Institute of Chinese Medicine
Email: info@benthamscience.net
Chuan Chen
, Shanghai Geriatric Institute of Chinese Medicine
Email: info@benthamscience.net
Dingzhu Shen
, Shanghai Geriatric Institute of Chinese Medicine
Email: info@benthamscience.net
Jiulin Chen
, Shanghai Geriatric Institute of Chinese Medicine
Email: info@benthamscience.net
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