Synthesis and antibacterial activity of silver nanoparticles stabilized by lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas fluorescens

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Abstract

The colloidal chemical and antibacterial properties of aqueous dispersions of silver nanoparticles stabilized by surfactin and rhamnolipids isolated from B. amyloliquefaciens and P. fluorescens were studied. The isolated biosurfactants were identified by thin layer chromatography and Fourier transform infrared spectrometry. Using the methods of UV-visible spectrophotometry, transmission electron microscopy and dynamic light scattering, the colloidal chemical characteristics of the resulting dispersions were studied. Optimal ratios of the precursor substances were found in which the used biosurfactants perform as effective stabilizers of dispersions of silver nanoparticles and ensure their aggregative stability for at least two months. It was shown that the studied dispersions have antibacterial activity against gram-positive B. subtilis and gram-negative P. aeruginosa and E. coli. A comparative assessment of the antibacterial activity of silver nanoparticles stabilized by biosurfactants and traditional silver-containing preparations, such as a silver nitrate solution and a dispersion of silver nanoparticles stabilized by citrate, was carried out. Furthermore, dispersions stabilized with surfactin showed the highest antibacterial activity, comparable to the effect of a silver nitrate solution, which is associated with their good colloidal stability. In addition, high antibacterial activity of dispersions of silver nanoparticles stabilized with biosurfactants isolated from Bacillus and Pseudomonas bacteria against strains of the other genus was discovered. An explanation of the observed phenomenon is given and the prospects for its application in medicine are proposed.

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A. G. Khina

Lomonosov Moscow State University; Bauman Moscow State Technical University

Author for correspondence.
Email: alex_khina@inbox.ru

Lomonosov Moscow State University, Department of Chemistry

Russian Federation, Moscow, 119991; Mosccow, 105005

A. S. Gordeev

Kazan Federal University

Email: alex_khina@inbox.ru

Institute of Ecology, Biotechnology and Nature Management

Russian Federation, Kazan, 420008

L. R. Biktasheva

Kazan Federal University

Email: alex_khina@inbox.ru

Institute of Ecology, Biotechnology and Nature Management

Russian Federation, Kazan, 420008

D. M. Gorbunov

Lomonosov Moscow State University

Email: alex_khina@inbox.ru

Department of Chemistry

Russian Federation, Moscow, 119991

P. A. Kuryntseva

Kazan Federal University

Email: alex_khina@inbox.ru

Institute of Ecology, Biotechnology and Nature Management

Russian Federation, Kazan, 420008

G. V. Lisichkin

Lomonosov Moscow State University

Email: alex_khina@inbox.ru

Department of Chemistry

Russian Federation, Moscow, 119991

Yu. A. Krutyakov

Lomonosov Moscow State University; National Research Center “Kurchatov Institute”

Email: nrcki@nrcki.ru

Lomonosov Moscow State University, Department of Chemistry

Russian Federation, Moscow, 119991; Moscow, 123182

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Supplementary files

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2. Fig. 1. IR spectrum of biosurfactants produced by B. amiloliquofaciens (a) and P. fluorescens (b).

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3. Fig. 2. Electron microdiffraction pattern of a sample of silver NP dispersion stabilized with surfactin.

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4. Fig. 3. Absorption spectra of silver nanoparticle dispersions with different stabilizers: AgNP-surfactin (a), AgNP-rhamnolipid (b), AgNP-citrate (c).

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5. Fig. 4. TEM electron micrographs of silver nanoparticle dispersions with different stabilizers: AgNPs-surfactin (a), AgNPs-rhamnolipid (b), AgNPs-citrate (c).

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6. Fig. 5. Dispersion composition of silver nanoparticles with different stabilizers: AgNP-surfactin (a), AgNP-rhamnolipid (b), AgNP-citrate (c).

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7. Fig. 6. Antibacterial activity of ions and dispersions of silver NPs with different stabilizers against P. aeruginosa after 96 h: Ag+ — AgNO3 solution; Ag-Cit NPs — Ag-citrate; Ag-Sf NPs — Ag-surfactin; Ag-Rh NPs — Ag-rhamnolipid; Sf — surfactin; Rh — rhamnolipid.

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