Effect of cadmium ions on the content of ∆5-sterols in microdomains of the vacuolar membrane

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

The effect of 100 μM cadmium ions (Cd2+) on the content of ∆5-sterols in microdomains of the vacuolar membrane was studied. Cd2+ was shown to cause an increase in the content of sterols in the tonoplast microdomains of zone 2 of the sucrose gradient and a decrease in the sterol content in zone 4. The sum of free sterols was higher than the sum of sterol esters in both the tonoplast and microdomains of zones 2, 4, and 6. The composition of free sterols in microdomains was dominated by β-sitosterol, and in the tonoplast by stigmasterol. The composition of sterol esters in the tonoplast also showed a high content of stigmasterol and a high content of β-sitosterol and campesterol in zone 2 microdomains. Compared with controls not exposed to Cd2+, changes in the % content of ∆5-sterols were observed. The changes affected mainly sterols involved in the structural organization of the lipid bilayer. These results suggest that sterols of tonoplast microdomains may participate in the cell response to Cd2+.

全文:

受限制的访问

作者简介

I. Kapustina

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Email: nichka.g@bk.ru
俄罗斯联邦, Irkutsk, 664033

E. Spiridonova

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Email: nichka.g@bk.ru
俄罗斯联邦, Irkutsk, 664033

N. Ozolina

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Email: nichka.g@bk.ru
俄罗斯联邦, Irkutsk, 664033

T. Lipchanskaya

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

Email: nichka.g@bk.ru
俄罗斯联邦, Irkutsk, 664033

V. Gurina

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: nichka.g@bk.ru
俄罗斯联邦, Irkutsk, 664033

参考

  1. Goncharuk E.A., Zagoskina N.V. 2023. Heavy metals, their phytotoxicity, and the role of phenolic antioxidants in plant stress responses with focus on cadmium: Review. Molecules. 28 (9), 3921. https://doi.org/10.3390/molecules28093921
  2. Ежегодник. Загрязнение почв Российской Федерации токсикантами промышленного происхождения в 2023 году. Обнинск: ФГБУ «НПО «Тайфун». 2024. 121 c.
  3. Liu C., Wen L., Cui Y., Ahammed G.J., Cheng Y. 2024. Metal transport proteins and transcription factor networks in plant responses to cadmium stress. Plant Cell Rep. 43 (9), 218. https://doi.org/10.1007/s00299-024-03303-x
  4. Kerek E.M., Prenner E.J. 2016. Inorganic cadmium affects the fluidity and size of phospholipid based liposomes. Biochim. Biophys. Acta. 1858 (12), 3169–3181. https://doi.org/10.1016/j.bbamem.2016.10.005
  5. Du Y., Fu X., Chu Y., Wu P., Liu Y., Ma L., Tian H., Zhu B. 2022. Biosynthesis and the roles of plant sterols in development and stress responses. Int. J. Mol. Sci. 23, 2332. https://doi.org/10.3390/ijms23042332
  6. Ozolina N.V., Kapustina I.S., Gurina V.V., Nurminsky V.N. 2022. Role of tonoplast microdomains in plant cell protection against osmotic stress. Planta. 255 (3), 65. https://doi.org/10.1007/s00425-021-03800-3
  7. Ozolina N.V., Kapustina I.S., Gurina V.V., Spiridonova E.V., Nurminsky V.N. 2024. Influence of oxidative stress upon the lipid composition of raft structures of the vacuolar membrane. Rus. J. Plant Physiol. 71, 29. https://doi.org/10.1134/S102144372460449X
  8. Spiridonova E., Ozolina N., Nesterkina I., Gurina V., Nurminsky V., Donskaya L., Tretyakova A. 2019. Effect of cadmium on the roots of beetroot (Beta vulgaris L.). Int. J. Phytoremediation. 21 (10), 980–984. https://doi.org/10.1080/15226514.2019.1583722
  9. Lapshin N.K., Piotrovskii M.S., Trofimova M.S. 2021. Sterol extraction from isolated plant plasma membrane vesicles affects H+-ATPase activity and H+-transport. Biomolecules. 11 (12), 1891. https://doi.org/10.3390/biom11121891
  10. Саляев Р.К., Кузеванов В.Я., Хаптагаев С.Б., Копытчук В.Н. 1981. Выделение и очистка вакуолей и вакуолярных мембран из клеток растений. Физиол. растений. 28, 1295–1305.
  11. Ozolina N.V., Nesterkina I.S., Kolesnikova E.V., Salyaev R.K., Nurminsky V.N., Rakevich A.L., Martynovich E.F., Chernyshov M.Yu. 2013. Tonoplast of Beta vulgaris L. contains detergent-resistant membrane microdomains. Planta. 237, 859–871. https://doi.org/10.1007/s00425-012-1800-1
  12. Ozolina N.V., Nesterkina I.S., Gurina V.V., Nurminsky V.N. 2020. Non-detergent isolation of membrane structures from beet plasmalemma and tonoplast having lipid composition characteristic of rafts. J. Membr. Biol. 253 (5), 479–489. https://doi.org/10.1007/s00232-020-00137-y
  13. Котлова Е.Р., Сеник С.В., Кюхер Т., Шаварда А.Л., Кияшко А.А., Псурцева Н.В., Синютина Н.Ф., Зубарев Р.А. 2009. Изменение состава мембранных глицеро- и сфинголипидов в ходе развития поверхностей культуры Flammulina velutipes. Микробиология. 78 (2), 226–235.
  14. Malins D.C., Mangold H.K. 1960. Analysis of complex lipid mixtures by thin‐layer chromatography and complementary methods. J. American Oil Chemistʼs Society. 37, 576–578.
  15. Дударева Л.В., Семенова Н.В., Нохсоров В.В., Рудиковская Е.Г., Петров К.А. 2020. Компонентный состав фитостеринов надземной части хвоща пестрого Equisétum variegatum Schleich. ex. Web., произрастающего в cеверо-восточной Якутии. Химия растительного сырья. 2, 133–139. https://doi.org/10.14258/jcprm.2020025555
  16. Rozentsvet O.A., Bogdanova E.S., Nurminsky V.N., Nesterov V.N., Chernyshov M.Yu. 2023. Detergent-resistant membranes in chloroplasts and mitochondria of the halophyte salicornia perennans under salt stress. Plants. 12 (6), 1265. https://doi.org/10.3390/plants12061265
  17. Спиридонова Е.В., Капустина И.С., Гурина В.В., Семёнова Н.В. Озолина Н.В. 2024. Изучение влияния ионов меди на состав фитостеринов вакуолярной мембраны Beta vulgaris L. Известия вузов. Прикладная химия и биотехнология. 14 (1), 90–98. https://doi.org/10.21285/achb.902
  18. Nokhsorov V.V., Dudareva L.V., Semenova N.V., Sofronova V.E. 2023. The Composition and the content of D-5 sterols, fatty acids, and the activity of acyl-lipid desaturases in the shoots of ephedra monosperma, introduced in the botanical garden of the cryolithozone of Yakutia. Horticulturae. 9, 858. https://doi.org/10.3390/horticulturae9080858
  19. Валитова Ю.Н., Сулкарнаева А.Г., Минибаева Ф.В. 2016. Растительные стерины: многообразие, биосинтез, физиологические функции. Биохимия. 81 (8), 1050. https://doi.org/10.1134/S0006297916080046
  20. Shimada T.L., Shimada T., Okazaki Y., Higashi Y., Saito K., Kuwata K., Oyama K., Kato M., Ueda H., Nakano A., Ueda T., Takano Y., Hara-Nishimura I. 2019. High sterol ester is a key factor in plant sterol homeostasis. Nat Plants. 5 (11), 1154–1166. https://doi.org/10.1038/s41477-019-0537-2
  21. Yepes-Molina L., Carvajal M., Martínez-Ballesta M.C. 2020. Detergent resistant membrane domains in broccoli plasma membrane associated to the response to salinity stress. Int. J. Mol. Sci. 21 (20), 7694. https://doi.org/10.3390/ijms21207694
  22. Valitova J., Renkova A., Beckett R., Minibayeva F. 2024. Stigmasterol: An enigmatic plant stress sterol with versatile functions. Int. J. Mol. Sci. 25, 8122. https://doi.org/10.3390/ijms25158122
  23. Нестёркина И.С., Гурина В.В., Озолина Н.В., Нурминский В.Н. 2019. Изменение содержание стеринов тонопласта при осмотическом стрессе. Биол. мембраны. 36 (4), 301–304. https://doi.org/10.1134/S0233475519040108
  24. Озолина Н.В., Гурина В.В., Капустина И.С., Спиридонова Е.В., Нурминский В.Н. 2023. Сравнение изменений в содержании стеринов плазмалеммы и тонопласта при окислительном и осмотических стрессах. Биол. мембраны. 40 (2), 147–150. https://doi.org/10.31857/S0233475523020056
  25. Ренкова А.Г., Хабибрахманова В.Р., Валитова Ю.Н., Мухитова Ф.К., Минибаева Ф.В. 2021. Действие стрессовых фитогормонов на метаболизм стеринов Triticum aestivum L. Физиол. растений. 68 (3), 279–288. https://doi.org/10.31857/S0015330321020159

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. The sum of free ∆5-sterols and their esters in the tonoplast and in microdomains from zones 2, 4, and 6 of the sucrose gradient after high-speed centrifugation of the tonoplast exposed to 100 μM Cd2+. FS – free sterols, ES – sterol esters, ∑ – the sum of free sterols and sterol esters. Data are presented as M ± SE, n = 3–5, p < 0.05. To prove the presence of significant differences, one-way analysis of variance followed by multiple comparison of means using Fisher's Least Significant Difference (LSD) method – the method of grouping samples with the least significant difference. Different letters indicate significant differences between the data in each analyzed parameter (FS; ES; ∑).

下载 (186KB)
索引源数据
3. Fig. 2. Contents of individual ∆5-sterol species in the tonoplast and microdomains from zones 2, 4, and 6 of the sucrose gradient after high-speed centrifugation of the tonoplast exposed to 100 μM Cd2+. A – free sterols, B – sterol esters. Data are presented as M ± SE, n = 3–5, p < 0.05. To test for significant differences, we used one-way analysis of variance followed by multiple comparison of means using Fisher’s LSD (Least Significant Difference) method – the method of grouping samples with the least significant difference. Different letters indicate significant differences between the data in each analyzed parameter (cholesterol, campesterol, stigmasterol, β-sitosterol).

下载 (397KB)
索引源数据

版权所有 © The Russian Academy of Sciences, 2025