Bioecological and Phytochemical Characteristics of Hyssopus officinalis (Lamiaceae) Grown in the Donetsk Region
- Authors: Prykhodko S.A.1, Vinogradova N.A.1, Kustova O.K.1
-
Affiliations:
- Donetsk Botanical Garden
- Issue: Vol 61, No 3 (2025)
- Pages: 74-85
- Section: Biology of Resource Species
- URL: https://rjraap.com/0033-9946/article/view/695432
- DOI: https://doi.org/10.7868/S3034572325030055
- EDN: https://elibrary.ru/ygiugr
- ID: 695432
Cite item
Abstract
The article presents comprehensive biomorphological study of Hyssopus officinalis L. and phytochemical study of its raw materials. The value of H. officinalis plant raw materials in terms of medicinal use is determined by high content of flavonoids (0.72 %), tannins (6.3 %), anthocyanins (0.79 %), organic (12.7 %) and hydroxycinnamic acids (6.0 %). High antioxidant activity of H. officinalis was revealed. The efficiency of biologically active substances extraction from H. officinalis was assessed using various extractants and their concentrations. Distilled water and 30 % ethanol were the most effective solvents producing the highest yields of bioactive substances form medicinal plant raw materials. The obtained results show the relevance of H. officinalis studies and are useful for expanding the raw material source range in the Donetsk region to produce herbal preparations with anti-inflammatory, antioxidant and capillary-strengthening effects. Environmental and biological plasticity of plants associated with the natural and climatic conditions of the southern Russian steppe is shown.
About the authors
S. A. Prykhodko
Donetsk Botanical Garden
Author for correspondence.
Email: arina0vinogradova@yandex.com
Donetsk, Russia
N. A. Vinogradova
Donetsk Botanical Garden
Email: arina0vinogradova@yandex.com
Donetsk, Russia
O. K. Kustova
Donetsk Botanical Garden
Email: arina0vinogradova@yandex.com
Donetsk, Russia
References
- David B., Wolfender J. L., Dias D. A. 2018. The pharmaceutical industry and natural products: historical status and new trends. — Phytochem. Rev. 14(2): 299—315. https://doi.org/10.1007/s11101-014-9367-z
- Boyko N. N., Bondarev A. V., Zhilyakova E. T., Pisarev D. I., Novikov O. O. 2017. Phytodrugs, analysis of Russian Federation pharmaceutical market. — Research Results in Biomedicine. 3(4): 30—38. https://doi.org/10.18413/2313-8955-2017-3-4-30-38 (In Russian)
- Olenina N. G., Mikheeva N. S., Krutikova N. M. 2018. Specific aspects of benefit-risk evaluation of herbal medicinal products: analysis of registration dossiers. — The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products. 8(2): 84—91. https://doi.org/10.30895/1991-2919-2018-8-2-84-91 (In Russian)
- Bespalyko L. V., Kharchenko V. A., Shevchenko Y. P., Ushakova I. T. 2016. Common hyssop (Hyssopus officinalis L.). — Vegetable Crops of Russia. 2(31): 60—63. https://elibrary.ru/wfgiwb (In Russian)
- Glukhov A. Z., Gorlacheva Z. S., Kustova O. K. 2013. [Essential oil plants (introduction, adaptive strategy, assessment of the prospects of cultivation)]. Donetsk. 238 p. (In Russian)
- Ahmadian S., Kenari R. E., Amiri Z. R., Sohbatzadeh F., Khodaparast M. H. H. 2023. Effect of ultrasound-assisted cold plasma pretreatment on cell wall polysaccharides distribution and extraction of phenolic compounds from hyssop (Hyssopus officinalism L.). — Int. J. Biol. Macromol. 233(1): 123557. https://doi.org/10.1016/j.ijbiomac.2023.123557
- Fathiazad F., Hamedeyazdan S. 2011. A review on Hyssopus officinalis L.: composition and biological activities. — Afr. J. Pharm. Pharmacol. 5(17): 1959—1966. https://doi.org/10.5897/AJPP11.527
- Mićović T., Topalović D., Živković L., Spremo-Potparević B., Jakovljević V., Matić S., Popović S., Baskić D., Stešević D., Samardžić S., Stojanović D., Maksimović Z. 2021. Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae). — Plants. 10(4): 711. https://doi.org/10.3390/plants10040711
- Mićović T., Stanković J. S. K., Bauer R., Nöst X., Marković Z., Milenković D., Jakovljević V., Tomović M., Bradić J., Stešević D., Stojanović D., Maksimović Z. 2022. In vitro, in vivo and in silico evaluation of the anti-inflammatory potential of Hyssopus officinalis L. subsp. aristatus (Godr.) Nyman (Lamiaceae). — J. Ethnopharmacol. 293: 115201. https://doi.org/10.1016/j.jep.2022.115201
- Skrypnik L., Feduraev P., Styran T., Golovin A., Katserov D., Nebreeva S., Maslennikov P. 2022. Biomass, phenolic compounds, essential oil content, and antioxidant properties of hyssop (Hyssopus officinalis L.) grown in hydroponics as affected by treatment type and selenium concentration. — Horticulturae. 8(11): 1037. https://doi.org/10.3390/horticulturae8111037
- Mashanov V. I., Pokrovskij A. A. 1991. [Spicy aromatic herbs]. Moscow. 287 p. (In Russian)
- Vinokurova O. A., Trineeva O. V., Slivkin A. I. 2016. Comparative characteristics of different types of thyme: the composition, properties and application (Review). — Drug Development and Registration. 4(17): 134—150. https://elibrary.ru/tartxx (In Russian)
- Vinogradova N., Vinogradova E., Chaplygin V., Mandzhieva S., Kumar P., Rajput V. D., Minkina T., Seth C. S., Burachevskaya M., Lysenko D., Singh R. K. 2023. Phenolic compounds of the medicinal plants in an anthropogenically transformed environment. — Molecules. 28(17): 6322. https://doi.org/10.3390/molecules28176322
- Malankina E. L., Tkacheva E. N., Kozlovskaya L. N. 2018. Medicinal plants of the Lamiaceae family as flavonoids sources. — Problems of Biological, Medicinal and Pharmaceutical Chemistry. 21(1): 30—35. https://doi.org/10.29296/25877313-2018-01-06 (In Russian)
- [Catalogue of plants of Donetsk Botanical Garden: Reference manual]. 1988. Kiev. 528 p. (In Russian)
- Pogotskaya A. A., Yorshik O. A., Buzuk G. N., Kuz’micheva N. A. 2014. [Guidelines for pharmacognostic practice]. Vitebsk. 66 p. https://elib.vsmu.by/handle/123/6973 (In Russian)
- [The State Pharmacopoeia of the Russian Federation XIV]. 2018. Vol. 4. Moscow. 1883 p. https://femb.ru/record/pharmacopea14 (In Russian)
- [The State Pharmacopoeia of the Russian Federation XV]. 2023. https://femb.ru/record/pharmacopea15 (In Russian)
- Sen’ T. V. 2006. [Pharmacognostic study of Hyssopus officinalis: Abstr. … Dis. Cand. (Farm.) Sci.]. Kursk. 25 p. (In Russian)
- Sirota T. V. Method of assessing antioxidant activity of superoxidedismutase and chemical compounds: Pat. 2144674, № 99103192/14; Appl. 24.02.1999; Publ. 20.01.2000 (In Russian)
- Libus’ O. K., Rabotyagov V. D., Kut’ko S. P., Khlypenko L. A. 2004. [Essential and aromatic herbs]. Kherson. 272 p. (In Russian)
- Ahmadi H., Babalar M., Sarcheshmeh M. A. A., Morshedloo M. R., Shokrpour M. 2020. Effects of exogenous application of citrulline on prolonged water stress damages in hyssop (Hyssopus officinalis L.): Antioxidant activity, biochemical indices, and essential oils profile. — Food Chem. 333: 127433. https://doi.org/10.1016/j.foodchem.2020.127433
- Kustova O. K., Glukhov A. Z., Kozub-Ptitsa V. V., Vinogradova N. A. 2024. [Prospects for growing medicinal and aromatic plants in the Azov region]. — In: Strategic problems, threats and risks of Azov Basin and the Sea of Azov region (“Hazardous Phenomena — V”): Proceedings of the V International Scientific Conference. Rostov-on-Don. P. 376—380. https://elibrary.ru/vlbjet (In Russian)
- Jordanov D., Nikolov P., Bojchinov A. 1970. [Phytotherapy. Treatment with medicinal herbs]. Sofia. 342 p. Transl. from Bulgarian. (In Russian)
- Lebeda A. F., Dzhurenko N. I., Isajkina A. P., Sobko V. G. 2004. [Medicinal plants. The most complete encyclopedia]. Moscow. 912 p. (In Russian)
- Shreter G. K. 1972. [Medicinal plants and plant raw materials included in the national pharmacopoeias]. Moscow. 120 p. (In Russian)
- Plugatar Y. V., Bulavin I. V., Ivanova N. N., Miroshnichenko N. N., Saplev N. M., Shevchuk O. M., Feskov S. A., Naumenko T. S. 2023. Study of the component composition of essential oil, morphology, anatomy and ploidy level of Hyssopus officinalis L. cyaneus Alef. — Horticulturae. 9(4): 480. https://doi.org/10.3390/horticulturae9040480
- Velikorodov A. V., Kovalev V. B., Kurbanova F. Kh., Shchepetova E. V. 2015. Chemical composition of essential oil of Hyssopus officinalis L., cultivated in the Astrakhan region. — Khimija Rastitel’nogo Syr’ja. 3: 71—76. https://doi.org/10.14258/jcprm.201503749 (In Russian)
- Kovalenko N. A., Akhramovich T. I., Supichenko G. N., Sachivko T. V., Bosak V. N. 2019. Antibacterial activity of Hyssopus officinalis essential oils. — Khimija Rastitel’nogo Syr’ja. 1: 191—199. https://doi.org/10.14258/jcprm.2019014083 (In Russian)
- Tonkovtseva V. V., Yarosh A. M., Batura I. A., Nagovskaya E. V., Koval E. S., Borkuta M. A., Grigoriev P. E. 2021. Impact of essential oil of Hyssopus officinalis on the psycho-emotional state and mental capacity of the elderly. — Scientific notes of V. I. Vernadski Crimean University. Biology. Chemistry. 7(2): 167—178. https://doi.org/10.37279/2413-1725-2021-7-2-167-178 (In Russian)
- Babich O., Sukhikh S., Pungin A., Astahova L., Chupakhin E., Belova D., Prosekov A., Ivanova S. 2021. Evaluation of the conditions for the cultivation of callus cultures of Hyssopus officinalis regarding the yield of polyphenolic compounds. — Plants. 10(5): 915. https://doi.org/10.3390/plants10050915
- Grebennikova O. A., Paliy A. E., Khlypenko L. A., Rabotyagov V. D. 2017. Biologically active substances of Hyssopus officinalis L. — The Orbital. 1: 21—28. https://elibrary.ru/zmsgwb (In Russian)
- Eshboev F., Karakozova M., Abdurakhmanov J., Bobakulov K., Dolimov K., Abdurashidov A., Baymirzaev A., Makhnyov A., Terenteva E., Sasmakov S., Piyakina G., Egamberdieva D., Nazarov P. A., Azimova S. 2023. Antimicrobial and cytotoxic activities of the secondary metabolites of endophytic fungi isolated from the medicinal plant Hyssopus officinalis. — Antibiotics. 12(7): 1201. https://doi.org/10.3390/antibiotics12071201
- Potoroko I., Kalinina I., Naumenko N., Fatkullin R., Shaik S., Sonawane S., Ivanova D., Kiselova-Kaneva Y., Tolstykh O., Paymulina A. 2017. Possibilities of regulating antioxidant activity of medicinal plant extracts. — Human. Sport. Medicine. 17(4): 77—90. https://doi.org/10.14529/hsm170409
- Olszewska M. 2008. Separation of quercetin, sexangularetin, kaempferol and isorhamnetin for simultaneous HPLC determination of flavonoid aglycones in inflorescences, leaves and fruits of three Sorbus species. — J. Pharm. Biomed. Anal. 48(3): 629—635. https://doi.org/10.1016/j.jpba.2008.06.004
- Sirota T. V. 2020. A chain reaction of adrenaline autoxidation is a model of quinoid oxidation of catecholamines. — Biophysics. 65(4): 646—655. https://doi.org/10.31857/S0006302920040031 (In Russian)
Supplementary files



