The effects of submicron particles of metal oxides on the production of hydrogen peroxide and the activity of oxidative enzymes of Aspergillus niger and Penicillium chrysogenum

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Abstract

The submicron particles effect of heavy metal oxides WO3, CsTeMoO6 и RbTe1.5W0.5O6 with photocatalytic activity on the content of hydrogen peroxide and the activity of extracellular oxidoreductases (catalase, peroxidase) in the cultivation medium of the Aspergillus niger and Penicillium chrysogenum fungi was studied. Addition of the studied compounds to the cultivation medium reduced the H2O2 content for both fungi. An ambiguous effect of the studied compounds on the activity of extracellular catalase and peroxidase was noted. In most cases, these compounds caused an increase in the activity of the studied enzymes both under light and in the dark. A significant decrease in activity was shown only for exocatalases of both fungi under the influence of WO3 and under the influence of CsTeMoO6 in P. chrysogenum.

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About the authors

N. А. Anikina

Lobachevsky Nizhegorod State University

Author for correspondence.
Email: undinaf@gmail.com
Russian Federation, Nizhny Novgorod

R. V. Baryshkov

Lobachevsky Nizhegorod State University

Email: romanbariskov1000@mail.ru
Russian Federation, Nizhny Novgorod

A. Yu. Shishkin

Lobachevsky Nizhegorod State University

Email: uandshi@yandex.ru
Russian Federation, Nizhny Novgorod

O. N. Smirnova

Lobachevsky Nizhegorod State University

Email: protectfun@mail.ru
Russian Federation, Nizhny Novgorod

V. F. Smirnov

Lobachevsky Nizhegorod State University

Email: biodeg@mail.ru
Russian Federation, Nizhny Novgorod

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2. Fig. 1. The content of hydroperoxides in the cultivation medium of Aspergillus niger (K) and when submicron particles of metal oxides WO3 (1), RbTe1.5W0.5O6 (2), CsTeMoO6 (3) are added to it under conditions of exposure to light (C) and in darkness (T).

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3. Fig. 2. The content of hydroperoxides in the cultivation medium of Penicillium chrysogenum (K) and when submicron particles of metal oxides WO3 (1), RbTe1.5W0.5O6 (2), CsTeMoO6 (3) are added to it under conditions of exposure to light (C) and in darkness (T).

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4. Fig. 3. Activity of extracellular catalase in the culture liquid of Aspergillus niger (K) and when submicron particles of metal oxides WO3 (1), RbTe1.5W0.5O6 (2), CsTeMoO6 (3) are added to it under conditions of exposure to light (C) and under conditions darkness (T).

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5. Fig. 4. Activity of extracellular catalase in the culture liquid of Penicillium chrysogenum (K) and when submicron particles of metal oxides WO3 (1), RbTe1.5W0.5O6 (2), CsTeMoO6 (3) are added to it under conditions of exposure to light (C) and under conditions darkness (T).

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6. Fig. 5. Activity of extracellular peroxidase in the culture liquid of Aspergillus niger (K) and when submicron particles of metal oxides WO3 (1), RbTe1.5W0.5O6 (2), CsTeMoO6 (3) are added to it under conditions of exposure to light (C) and under conditions darkness (T).

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7. Fig. 6. Activity of extracellular peroxidase in the culture liquid of Penicillium chrysogenum (K) and when submicron particles of metal oxides WO3 (1), RbTe1.5W0.5O6 (2), CsTeMoO6 (3) are added to it under conditions of exposure to light (C) and under conditions darkness (T).

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