Influence of single-walled carbon nanotubes ingestion by rats on their integral and biochemical indices

Cover Page

Cite item

Full Text

Abstract

Introduction. Single-walled carbon nanotubes (SWCNTs) can affect the human body as a result of their use in packaging materials, growth stimulators of agricultural plants and promising agrochemicals. 

The aim of the study was to investigate the effect of SWCNT on integral and biochemical indices on the model of oral administration to rats in a subacute experiment with duration of 92 days.

Material and methods. SWCNT were dispersed by ultrasound in water with 1% by weight of the non-ionic surfactant Tween 20. According to the data of dynamic light scattering, electron microscopy, and Raman light scattering the sample containing both free and partially aggregated SWCNTs. The experiment was carried out on 5 groups of growing male Wistar rats. The SWCNT dispersion was added to the drinking water at doses (control); of 0.01; 0.1; 1.0 and 10 mg/kg body weight in groups from the 1st to the 5th group. We studied the level of anxiety and cognitive function in the test of the “Conditional Reflex of Passive Avoidance” (CRPA); the mass of internal organs, the permeability of the small intestine wall for ovalbumin macromolecules, the biochemical parameters of blood serum, the activity of glutathione peroxidase, the content of non-protein thiols in the liver, urinary excretion of 8-hydroxy-2’-deoxyguanosine (8-oxo-G) and selenium.

Results. As a result of SWCNTs consumption there were found significant changes in the content of total protein, uric acid, triglycerides, cholesterol in the blood serum, the increased activity of aspartate aminotransferase, the relative mass of the liver, and decreased the permeability of the intestinal wall for macromolecules of the protein. There have been shifts in individual antioxidant protection indices, including increased 8-oxo-G excretion, decreased glutathione peroxidase activity, glutathione content in the liver, and selenium availability. Most of the above effects did not show a dose dependence of the nanomaterial and were more pronounced at minimal and medium doses of SWCNTs than at maximum doses. The effect of SWCNTs on the behavioral responses of animals was not revealed. 

Conclusion. Therefore, SWCNT show signs of toxic effects at a dose of 0.1 mg/kg body weight in a day or less.

About the authors

V. A. Shipelin

Federal Research Centre for Nutrition and Biotechnology

Author for correspondence.
Email: noemail@neicon.ru
ORCID iD: 0000-0002-0015-8735
Russian Federation

A. A. Shumakova

Federal Research Centre for Nutrition and Biotechnology

Email: noemail@neicon.ru
ORCID iD: 0000-0003-1373-4436
Russian Federation

Kh. S. Soto

Federal Research Centre for Nutrition and Biotechnology

Email: noemail@neicon.ru
ORCID iD: 0000-0001-6622-5251
Russian Federation

A. V. Selifanov

Federal Research Centre for Nutrition and Biotechnology

Email: noemail@neicon.ru
Russian Federation

Yu. S. Sidorova

Federal Research Centre for Nutrition and Biotechnology

Email: noemail@neicon.ru
Russian Federation

A. G. Masyutin

Lomonosov Moscow State University

Email: noemail@neicon.ru
Russian Federation

A. I. Chernov

Prokhorov General Physics Institute of the Russian Academy of Sciences

Email: noemail@neicon.ru
ORCID iD: 0000-0002-6492-9068
Russian Federation

Ivan V. Gmoshinski

Federal Research Centre for Nutrition and Biotechnology

Email: gmosh@ion.ru
ORCID iD: 0000-0002-3671-6508

MD, Ph.D., DSci., D.Sc, Ph.D. in Biology, leading researcher of the Department of Food Toxicology and Evaluation of Nanotechnology Safety, Federal Research Centre for Nutrition and Biotechnology, Moscow, 109240, Russian Federation.

e-mail: gmosh@ion.ru

Russian Federation

S. A. Khotimchenko

Federal Research Centre for Nutrition and Biotechnology; I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: noemail@neicon.ru
ORCID iD: 0000-0002-5340-9649
Russian Federation

References

  1. De Volder M.F., Tawfick S.H., Baughman R.H., Hart A.J. Carbon nanotubes: present and future commercial applications. Science. 2013; 339 (6119): 535-9. https://doi.org/10.1126/science.1222453
  2. Coyuco J.C., Liu Y., Tan B.-J., Chiu G.N.C. Functionalized carbon nanomaterials: exploring the interactions with Caco-2 cells for potential oral drug delivery. Int. J. Nanomedicine. 2011; 6: 2253-63.
  3. Patlolla A., McGinnis B., Tchounwou P. Biochemical and histopathological evaluation of func-tionalized single-walled carbon nanotube in Swiss-Webster mice. J. Appl. Toxicol. 2011; 31(1): 75–83.
  4. Shvedova A.A., Kisin E.R., Mercer R., Murray A.R., Johnson V.J., Potapovich A.I. et al. Un-usual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice. Am. J. Physiol. Lung Cell. Mol. Physiol. 2005; 289 (5): 698-708.
  5. Kolosnjaj-Tabi J., Hartman K.B., Boudjemaa S., Ananta J.S., Morgant G., Szwarc H. et al. In vivo behavior of large doses of ultrashort and full-length single-walled carbon nanotubes after oral and intraperitoneal administration to Swiss mice. ACS Nano. 2010; 4 (3): 1481-92.
  6. Khripach L.V., Rakhmanin Yu.A., Mikhailova R.I., Knyazeva T.D., Koganova Z.I., Zheleznyak E.V. et al. Biochemical effects of chronic peroral administration of carbon nanotubes and activatyed charcoal in drinking water in rats. Gigiena i sanitariya. 2014; 5: 36-42. (in Russian)
  7. Philbrook N.A., Walker V.K., Afrooz A.R., Saleh N.B., Winn L.M. Investigating the effects of functionalized carbon nanotubes on reproduction and development in Drosophila melanogaster and CD-1 mice. Reprod. Toxicol. 2011; 32 (4): 442-8.
  8. Li P., Lai X., Witzmann F.A., Blazer-Yost B.L. Bioinformatic analysis of differential protein expression in Calu-3 cells exposed to carbon nanotubes. Proteomes. 2013; 1 (3): 219-39.
  9. Shipelin V.A., Shumakova A.A., Masyutin A.G., Chernov A.I., Sidorova Yu.S., Gmoshinski I.V. et al. In vivo subacute oral toxicity assessment of multiwalled carbon nanotubes: characteristic of nanomaterial and integral indicators. Nanotechnologies in Russia. 2017; 12(9-10): 559-568. https://doi.org/10.1134/S199507801705010X
  10. Order of the Ministry of Health of the Russian Federation No. 199n of 01/01/2016 “On approval of the rules of good laboratory practice”.
  11. Guide for the care and use of laboratory animals. Eighth Edition. Committee for the Update of the Guide for the Care and Use of Laboratory Animals; Institute for Laboratory Animal Research (ILAR); Division on Earth and Life Studies (DELS); National Research Council of the national academies. Washington: The National Academies Press. 2011.
  12. Loskutova L.V., Dubrovina N.I., Markel' A.L. Comparative analysis of the persistence of a conditioned passive avoidance reflex in rats with different forms of inherited hypertension. Neurosci. Behav. Physiol. 2007; 37 (6): 577-82.
  13. Razygraev A.V. Method for determination of glutathione peroxidase activity using hydrogen peroxide and 5,5'-dithiobis (2-nitrobenzoic acid). Kliniko-laboratornyy konsilium. 2004; 4: 19-22. (In Russian)
  14. Golubkina N.A. Fluorimetric method for the determination of selenium. Zhurn. analiticheskoy khimii. 1995; 50(8): 492-497. (In Russian)
  15. Udall J.N., Pang K., Fritze L., Kleinman R., Walker W.A. Development of gastrointestinal mucosal barrier. I. The effect of age on intestinal permeability to macromolecules. Pediat. Res. 1981; 15(3): 241-4.
  16. Polyoxyethylene (20) sorbitan monoesters of lauric, oleic, palmitic and stearic acid and triester оf stearic acid. Toxicological evaluation of some food additives including anticaking agents, antimicrobials, antioxidants, emulsifiers and thickening agents. WHO Food Additives Series. 1974; 5: 8. Электронный ресурс: http://www.inchem.org
  17. Ju L., Zhang W., Wang X., Hu J., Zhang Y.: Aggregation kinetics of SDBSdispersed carbon nanotubes in different aqueous suspensions. Colloids and Surfaces A: Physicochemical and En-gineering Aspects. 2012; 409: 159-66.
  18. Ferguson P.L., Chandler G.T., Templeton R.C., Demarco A., Scrivens W.A., Englehart B.A. Influence of sediment-amendment with single-walled carbon nanotubes and diesel shoot on bioaccumulation of hydrophobic organic contaminats by bentic invertebrates. Environ. Sci. Technol. 2008; 42 (10): 3879.
  19. Gorsheneva E.B. Dose-dependent effect of multi-walled carbon nanotubes and soot particles when administered orally to laboratory mice. Nanotekhnologii i okhrana zdorov'ya. 2014; 6(18): 48-55. (In Russian)
  20. Chen G., Chen R., Zou C., Yang D., Chen Z-S. Fragmented polymer nanotubes from sonication-induced scission with a thermo-responsive gating system for anti-cancer drug delivery. J. Mater. Chem. B. 2014; 10(2): 1327-34.
  21. Zaragoza-Contreras E.A., Lozano-Rodríguez E.D., Román-Aguirre M., Antunez-Flores W., Hernández-Escobar C.A., Flores-Gallardo S.G. et al. Evidence of multi-walled carbon nanotube fragmentation induced by sonication during nanotube encapsulation via bulk-suspension polymerization. Micron. 2009; 40 (5–6): 621-7.
  22. Shumakova A.A., Gmoshinsky I.V., Shipelin V.A., Rezaeva D.M., Khotimchenko S. A. Effect of multiwalled carbon nanotubes on the microelement status in the internal organs of rats in an experiment. Nanotechnologies in Russia. 2018; 13 (3–4): 189–94.
  23. Jachak A., Lai S.K, Hida K., Suk J.S., Markovic N., Biswal S., et al. Transport of metal oxide nanoparticles and single-walled carbon nanotubes in human mucus. Nanotoxicology. 2012; 6 (6): 614-22.
  24. Pauluhn J. Subchronic 13-week inhalation exposure of rats to multiwalled carbon nanotubes: toxic effects are determined by density of agglomerate structures, not fibrillar structures. Toxicol Sci. 2010; 113(1): 226-42.
  25. Zhang Z.P., Tian Y.H., Li R., Cheng X.Q., Guo S.M., Zhang J.X. et al. The comparison of the normal blood biochemical values of Wistar rats with different age and sex. Asian Journal of Drug Metabolism and Pharmacokinetics. 2004; 4: 215-8.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Shipelin V.A., Shumakova A.A., Soto K.S., Selifanov A.V., Sidorova Y.S., Masyutin A.G., Chernov A.I., Gmoshinski I.V., Khotimchenko S.A.


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 37884 от 02.10.2009.