The effect of vitamins a and e on the structure of the small intestine of rats after two weeks and then three months following exposure to single-walled carbon nanotubes

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Abstract

Introduction. Currently, there is a number of works aimed at studying the effect of carbon nanotubes (UNT), including single-walled (oUNT), on the body and there are studies to find means to reduce their toxic effects, such as compounds with antioxidant properties.

Material and methods. In fifteen groups of animals there were studied intragastric effects of two weeks with the count at doses of 0.05 and 0.5 mg/kg and a single dose of 5mg/kg, as well as complex with vitamins A and E (AE) at a dose of 25.3 mg/kg on the small intestine of male Wistar rats, and in the same batch of animals three months later.

Results. In a two-week experiment under the influence of oUNT in doses of 0.05 and 0.5 mg/kg there was shown the dose dependence on indices of reducing the number of normal villi with an increase in the number of destructured ones, whereas three months later among the destructured villi were increased in number with apical necrosis. After a single exposure of oUNT at a dose of 5mg/kg three months later, there also was increased the number of villi with apical necrosis. Two-weeks exposure of AE reduced the number of normal villi, increasing the number of degraded forms, and three months later the number of villi with apical necrosis increased. Two-weeks complex exposure of AE and oUN at doses of 0.05 and 0.5 mg/kg reduced the number of normal villi with an increasing number of destructured ones; three months later, as well as with other effects, the pathology developed, increasing the number of villi with apical necrosis.

About the authors

Natalia N. Belyaeva

F.F. Erisman Federal Research Center of Hygiene

Author for correspondence.
Email: belnatnik@mail.ru
ORCID iD: 0000-0003-2504-9815

MD, Ph.D., DSci., leading researcher of the Department of toxicology and environmental hygiene of the F.F. Erisman Federal Research Center of Hygiene, Mytishchi, 141014, Russian Federation.

e-mail: belnatnik@mail.ru

Russian Federation

M. V. Vostrikova

F.F. Erisman Federal Research Center of Hygiene

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4090-5202
Russian Federation

N. N. Demina

Centre for Strategic Planning, Russian Ministry of Health

Email: noemail@neicon.ru
Russian Federation

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