Change of the profile of expression of adaptive response genes in toxic hepatitis of different etiology

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Abstract

Introduction. In Russia, the problem of toxic hepatitis is of great importance and relevance. The etiology of toxic hepatitis (industrial toxicants, drugs, ethanol) and, as a consequence, pathogenesis may have significant differences at the molecular genetic level.

The aim of the study was to analyze the expression of genes involved in the response to toxic liver damage of various etiologies.

Material and methods. Toxic hepatitis was modulated in male albino mongrel rats weighing 180-200 grams assigned to four groups (control group, carbon tetrachloride, paracetamol, ethanol). After 24 and 72 hours of paracetamol administration, rats were anesthetized and the mRNA levels of the Chek1, Gclc, Gstm1, Gstp1, Gstt1, Nfe2l2, Nqo1, Ripk1 genes in the liver homogenate were examined.

Results. As a result of the analysis of the genes expression studied, the expression profile was found TO be differed depending on the etiology of toxic hepatitis. With carbon tetrachloride poisoning, an increase in the expression of the Nqo1 genes (p = 0.001), Gstm1 (p = 0.037) and a decrease in the expression of the Nfe2l2 genes (p = 0.004), Ripk1 (p = 0.004) was observed. With the liver damage by paracetamol and its metabolites, opposite to the expression of the Gstm1 gene (p = 0.001) decreased, and the expression of the Nfe2l2 (p = 0.009), Gclc (p = 0.001), Chek1 (p = 0.011) genes increased. During alcohol intoxication, there were no statistically significant changes in the expression profiles of the genes studied.

Conclusion. the results obtained may indicate the involvement of various molecular genetic mechanisms in the process of response to toxic liver damage, depending on the etiology.

About the authors

Denis O. Karimov

Ufa Research Institute of Occupational Health and Human Ecology

Author for correspondence.
Email: karimovdo@gmail.com

MD, Ph.D., head of the department of toxicology and genetics with an experimental clinic of laboratory animals professor, head of Department of General Hygiene of Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation.

e-mail: karimovdo@gmail.com

Russian Federation

T. G. Kutlina

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
Russian Federation

G. F. Mukhammadiyeva

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
Russian Federation

Y. V. Valova

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
Russian Federation

E. F. Repina

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
Russian Federation

N. Yu. Khusnutdinova

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2024 Karimov D.O., Kutlina T.G., Mukhammadiyeva G.F., Valova Y.V., Repina E.F., Khusnutdinova N.Y.


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