Microbiological quality control of wastewater by species identification of microorganisms using MALDI-TOF MS

Introduction. One of the main tasks of medical care for infectious diseases is the rapid identification of an infectious agent.

The purpose of the study is to evaluate the effectiveness of the MALDI mass spectrometry for identification bacteria for microbiological control of wastewater quality.

Materials and methods. Samples of wastewater samples at the treatment stage from the Kuryanovskaya aeration station were analyzed in accordance with MU 2.1.5.800-99 “Management of state sanitary and epidemiological supervision of wastewater disinfection” by the identification method in accordance with MUK 4.2.1884-04 “Sanitary-microbiological and sanitary — parasitological water analysis of surface water bodies” with application MALDI-TOF MS and 16S rRNA gene sequencing.

Results. 5 strains of museum reference cultures and 22 bacterial isolates from wastewater samples grown on selective media of Endo, Enterococcus and Chromococcus coliform agar were studied, identified by MALDI-TOF MS, and confirmed by sequencing of specific regions of the 16S rRNA gene in bacteria of the genus Salmonella by MALDI-TOF MS identified only gender.

Conclusion. In the routine practice of microbiological research, the identification of microorganisms is based on the determination of their cultural, tinctorial properties, and biochemical activity, the determination of which requires large financial and time costs. The use of the MALDI-TOF MS method makes it possible to reduce the time of identification of a microorganism when visible growth of microorganisms appears.

Contribution:
Streletskiy A.V., Sukhina M.A., Vodianova M.A., Zagainova A.V. — study concept and design, statistical processing, text writing, editing;
Avtonomova A.V. — statistical processing, text writing, editing;
Ekaterincheva E.S., Tolkacheva L.R., Gritsyuk O.V., Novozhilov K.A. — collection and processing of material, experimental work, statistical processing, text writing, editing.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: March 15, 2022 / Accepted: April 21, 2022 / Published: May 31, 2022