Polymorphism of HIF-1A (rs11549465) and VEGFA (rs2010963) genes and the immune status in the dust lung pathology miners working at the coal enterprises in the South of Kuzbass

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Abstract

The aim of the study was to study the polymorphism of HIF-1A (rs11549465) and VEGFA (rs2010963) genes and their association with immunological parameters among the miners with lung dust pathology in the Kemerovo region.

Material and methods. 200 Kuzbass miners aged from 39 to 58 years, working in the primary occupations with a high risk of occupational pathology, were examined. All the subjects were divided into two groups: the 1st group included 130 cases with a proven diagnosis of dust lung pathology. The 2nd group was a control one represented by 70 workers in the same sanitary and hygienic conditions but without occupational diseases. Polymorphisms of the HIF-1A (rs11549465) and VEGFА (rs2010963) genes were studied by the method of real-time polymerase chain reaction. Immunological parameters were determined by immunoenzyme (immunoglobulins A, M and G) and immunoturbidimetric (haptoglobin, ceruloplasmin and α-1-antitrypsin) methods.

Results. The study of the distribution of heterozygous genotypes 1772C/T HIF-1A and -634G/C VEGFA did not reveal statistically significant differences between the miners with dust lung pathology and healthy individuals in the South of the Kemerovo region. However, the study results showed heterozygous polymorphisms HIF-1A and VEGFA, particularly the level of haptoglobin α-1-antitrypsin, ceruloplasmin, and IgM, to affect the immune status of the miners with dust lung pathology.

Conclusion. Heterozygous polymorphisms 1772C/T HIF-1A and -634G/C VEGFA can be used to determine the immune response and predict the development of dust lung pathology in miners, as well as the choice of treatment and preventive measures.

Contribution:

Zhukova A.G. — the concept and design of the study, writing a text;

Kazitskaya A.S. — collection and processing of material, statistical processing, editing;

Yadykina T.K. — collection and processing of material;

Panev N.I. — editing.

All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Acknowledgement. The study had no sponsorship.

Conflict of interest. The authors declare no conflict of interest. 

Conclusion of the Biomedical Ethics Committee of the Research Institute for Complex Problems of Hygiene and Occupational Diseases: the study was carried out in accordance with the ethical standard of the Bioethical Committee of the Research Institute for Complex Problems of Hygiene and Occupational Diseases, developed in accordance with the Declaration of Helsinki of the World Association “Ethical principles of scientific research with human participation”, as amended in 2013 . and “Rules of Clinical Practice in the Russian Federation” approved by the Order of the Ministry of Health of the Russian Federation No. 266 of June 19, 2003.

About the authors

Anna G. Zhukova

Research Institute for Complex Problems of Hygiene and Occupational Diseases; Novokuznetsk Institute (Branch Campus) of the Kemerovo State University

Author for correspondence.
Email: nyura_g@mail.ru
ORCID iD: 0000-0002-4797-7842

MD, PhD, DSci., Associate Professor, head of the Laboratory of molecular-genetic and experimental studies of the Research Institute for Complex Problems of Hygiene and Occupational Diseases, Novokuznetsk, 654041, Russian Federation; head of the natural sciences sub-department of the Novokuznetsk Institute (Branch Campus) of the Kemerovo State University, Novokuznetsk, 654041, Russian Federation.

e-mail: nyura_g@mail.ru

Russian Federation

Anastasiya S. Kazitskaya

Research Institute for Complex Problems of Hygiene and Occupational Diseases

Email: noemail@neicon.ru
ORCID iD: 0000-0001-8292-4810
Russian Federation

Tatyana K. Yadykina

Research Institute for Complex Problems of Hygiene and Occupational Diseases

Email: noemail@neicon.ru
ORCID iD: 0000-0001-7008-1035
Russian Federation

Nikolay I. Panev

Research Institute for Complex Problems of Hygiene and Occupational Diseases

Email: noemail@neicon.ru
ORCID iD: 0000-0001-5775-2615
Russian Federation

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