Cytomic analysis: a modern universal tool for biomedical and ecological and hygienic research (literature review). Part 2

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Abstract

In the second part of the review, examples of cytome analysis on both peripheral blood lymphocytes and human buccal epiteliocytes are given in the study of occupational risks and investigation of genome instabilities induced by alcohol and tobacco use. Much attention is paid to the cytome analysis application in environmental and hygienic research and the studies directed to evaluating natural and anthropogenic load to the human population, especially industrial, factors. The method of cytome analysis on primary culture of human peripheral blood lymphocytes cultivated with a cytokinetic block (CBMN-cyt test) in its current state is described in detail, and the history of its development is briefly reflected. The method of cytome analysis on human buccal epiteliocytes (BMN-cyt) is also analyzed. The review contains photomicrographs of genetic cell damages that are markers in micronuclei (MN) tests. We used PubMed, Web of Science, ResearchGate, Scopus, eLibrary databases as the sources of literature.

Contribution:
Krivtsova E.K. — search of literature sources, analysis and interpretation of literature data, writing a text;
Ingel F.I. — the concept and design of the study, a search of literature sources;
Akhaltseva L.V. — search of literature sources, analysis and interpretation of literature data.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: April 27, 2021 / Accepted: September 28, 2021 / Published: October 31, 2021

About the authors

Elena K. Krivtsova

Centre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical Biological Agency

Author for correspondence.
Email: ekrivcova@cspmz.ru
ORCID iD: 0000-0002-5039-8980

MD, researcher of the Department of Preventive Toxicology and Biomedical Research in the Centre for Strategic Planning of the Federal Medical Biological Agency, Moscow, 119121, Russian Federation.

e-mail: EKrivcova@cspmz.ru

Russian Federation

Faina I. Ingel

Centre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical Biological Agency

Email: noemail@neicon.ru
ORCID iD: 0000-0002-2262-6800
Russian Federation

Lyudmila V. Akhaltseva

Centre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical Biological Agency

Email: noemail@neicon.ru
ORCID iD: 0000-0002-3619-3858
Russian Federation

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