Evaluation of the effectiveness of the use of hepatoprotector in acute liver damage by various toxicants in the experiment

Introduction. Acute liver damage with ethanol and its surrogates and chemicals remains an urgent problem. Therefore, studies of the use of hepatoprotector in acute liver damage by various toxicants in the experiment are relevant.

The purpose of this study is an experimental evaluation of the use of ademetionine in the early stages of the toxic effects of carbon tetrachloride and ethanol.

Materials and methods. The therapeutic effect of the drug “Heptor” in acute intoxication with carbon tetrachloride (subcutaneous administration at a dose of 2 g/kg) and ethanol (oral administration at a dose of 4 g/kg weight) was studied. Studies of metabolic processes in the liver were carried out based on biochemical parameters of rat blood serum.

Results. The studies showed that normalization of metabolic processes was observed after introducing “Heptor” against the background of exposure to both toxicants. The therapeutic effect of ademetionine in the case of carbon tetrachloride intoxication had a positive impact after 24 hours of administration and persisted after 72 hours of the experiment. These provisions were based on the results obtained: the restoration of the activity of marker enzymes of hepatocytes (AsAT, AlAT, LDH), the concentration of uric acid and cholesterol, and the indicators of protein metabolism were revealed. The introduction of ademetionine after ethanol intoxication helped restore the function of hepatocytes, which led to the normalization of protein metabolism. The drug stopped hyperenzymemia, which confirmed its membrane-protective properties.

Conclusion. “Heptor” has a regenerating, detoxifying and membrane-protective effect in acute liver lesions with carbon tetrachloride and ethanol. The obtained data confirm the universality of this drug, based on the possibility of using various mechanisms of therapeutic action, which allows us to recommend ademetionine as a hepatoprotector to prevent early liver damage when exposed to high doses of multiple toxicants.

Contribution:
Timasheva G.V. — concept and design of the study, collection and processing of material, statistical processing, writing text, editing;
Bakirov A.B. — concept and design of the study, editing;
Mukhammadieva G.F. — collection of literature data, editing;
Smolyankin D.A., Khusnutdinova N.Yu., Karimov D.D. — collection and processing of the material.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conclusion of the bioethical commission: the study was approved by the bioethical commission of the Ufa Research Institute of Occupational Medicine and Human Ecology, carried out according to the European Convention for the Protection of Vertebrate Animals Used for Experiments or Other Scientific Purposes (ETS N 123), the directive of the European Parliament and the Council of the European Union 2010/63/EU of 22.09.2010 on the protection of animals used for scientific purposes.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The work was carried out within the framework of the industry research program of Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing (Rospotrebnadzor) “Hygienic scientific justification for minimizing risks to the health of the population of Russia” for 2016-2020 on the topic 3.5 Reg. N R & D AAAA16-116022610045-4 Reg. N ICRBS.

Received: May 25, 2021 / Accepted: September 28, 2021 / Published: November 30, 2021