Biotesting for the objectivization of the hygienic evaluation of the surface and underground drinking water sources

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Abstract

Introduction. An algorithm for evaluating the hygienic safety of drinking water sources based on biotesting and studying sanitary and chemical indicators has been developed and tested.

Materials and methods. The research methods included sanitary and chemical analysis of water samples, as well as biotesting methods using single-celled green alga Chlorella vulgaris Beijer and bull spermatozoa.

Results. For experimental approbation of the developed algorithm for determining the hygienic safety of water based on a two-component system of express biotesting and measurement of sanitary and chemical indicators, 6 underground and 5 surface water sources were evaluated. The response of test objects indicating the presence of acute toxicity in water samples taken from surface and underground in many cases correlates with the excess of MPC for individual indicators identified by sanitary and chemical analysis. For two water samples, differences were found between the results of biotesting and sanitary-chemical analysis, and therefore it is necessary to conduct an extended toxicological and chemical assessment to identify the source of toxic effects.

Limitations. The two-component test system cannot be used separately without sanitary and chemical analysis of water media.

Conclusion. Biotesting can be an addition to sanitary and chemical analyses, showing the presence of toxic substances that are not in the list of mandatory indicators to be determined (according to MR 2.1.4.0176-20 (in Russian)).

Compliance with ethical standards. The study does not require the submission of a biomedical ethics committee opinion or other documents.

Contribution:
Gusev Yu.S. — concept and design of the study, writing the text, responsibility for the integrity of all parts of the article;
Ivanov D.E. — concept and design of the study, writing the text;
Erdniev L.P. — writing the text, editing;
Kuzyanov D.A. — collection and processing of material, statistical data processing, writing text, editing;
Kosheleva I.S. — collection and processing of material, writing text, editing;
Savina K.A. — collection and processing of material, writing text, editing;
Mikerov A.N. — concept and design of the study, approval of the final version of the article.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version. 

Conflict of interest. The authors declare no conflict of interest. 

Acknowledgement. The study had no sponsorship. 

Received: Octoober 10, 2022 / Accepted: December 8, 2022 / Published: January 12, 2023 

About the authors

Yurij S. Gusev

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Author for correspondence.
Email: yuran1989@yandex.ru
ORCID iD: 0000-0001-7379-484X

MD, PhD, researcher of Laboratory of chemical-biological monitoring of water quality of the Saratov Medical Scientific Centre of Hygiene of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Saratov, 410022, Russian Federation.

e-mail: yuran1989@yandex.ru

Russian Federation

Dmitrij E. Ivanov

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Saratov State Law Academy

Email: noemail@neicon.ru
ORCID iD: 0000-0001-8162-9019
Russian Federation

Leonid P. Erdniev

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: noemail@neicon.ru
ORCID iD: 0000-0001-5187-7361
Russian Federation

Dmitrij A. Kuzyanov

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: noemail@neicon.ru
ORCID iD: 0000-0002-5070-4431
Russian Federation

Irina S. Kosheleva

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: noemail@neicon.ru
ORCID iD: 0000-0003-1992-5305
Russian Federation

Kseniya A. Savina

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: noemail@neicon.ru
ORCID iD: 0000-0003-4878-8784
Russian Federation

Anatolij N. Mikerov

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Saratov State Medical University named after V.I. Razumovsky

Email: noemail@neicon.ru
ORCID iD: 0000-0002-0670-7918
Russian Federation

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Copyright (c) 2023 Gusev Y.S., Ivanov D.E., Erdniev L.P., Kuzyanov D.A., Kosheleva I.S., Savina K.A., Mikerov A.N.


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