Improvement of the process of cryopreservation of cattle sperm with molecular hydrogen

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The effect of molecular hydrogen on the functional parameters of bovine sperm cells has been studied. The influence of molecular hydrogen on motility, ATP content, oxidative processes, viability and morphology of the acrosome in bull spermatozoa has been studied. The study was performed on sperm production of black-and-white cattle. The sperm was diluted with a sterile BioXcell medium (France). To analyze the effect of molecular hydrogen on bull spermatozoa, molecular hydrogen was added to the BioXcell medium. The analyzed parameters were studied in native sperm diluted with BioXcell medium, in sperm after deep freezing, as well as sperm subjected to deep freezing and pretreatment with molecular hydrogen. The addition of molecular hydrogen to the medium for diluting sperm contributed to an increase in cell mobility, increased energy metabolism and reduced oxidative stress of spermatozoa. The results obtained showed the need for an in-depth study of the effect of molecular hydrogen on the qualitative characteristics of sperm in cattle and, on this basis, to clarify the existing technological regulations for seed conservation.

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M. Ivashchenko

National Research Nizhny Novgorod State University named after N.I. Lobachevsky; Nizhny Novgorod State Agrotechnological University named after L.I. Florentyev

编辑信件的主要联系方式.
Email: kafedra2577@mail.ru

PhD in Biological Sciences

俄罗斯联邦, Nizhny Novgorod; Nizhny Novgorod

A. Deryuginа

National Research Nizhny Novgorod State University named after N.I. Lobachevsky

Email: kafedra2577@mail.ru

Grand PhD in Biological Sciences

俄罗斯联邦, Nizhny Novgorod

А. Belov

National Research Nizhny Novgorod State University named after N.I. Lobachevsky; Nizhny Novgorod State Agrotechnological University named after L.I. Florentyev

Email: kafedra2577@mail.ru

PhD in Biological Sciences

俄罗斯联邦, Nizhny Novgorod; Nizhny Novgorod

М. Latushko

Production Association “Ural Optical and Mechanical Plant” named after E.S. Yalamov

Email: kafedra2577@mail.ru

PhD in Technical Sciences

俄罗斯联邦, Yekaterinburg

P. Ignatiev

Production Association “Ural Optical and Mechanical Plant” named after E.S. Yalamov

Email: kafedra2577@mail.ru

PhD in Physical and Mathematical Sciences

俄罗斯联邦, Yekaterinburg

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