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Fig. 3. SDS-PAGE and enzyme immunoassay of bsNT aHA-l2-E7(aIgA), also referred to as (HN-E7)x3. a – Electrophoretic separation in a 5-19% gradient SDS polyacrylamide gel of affine purified bsNT, initial (1), overshoot on a filter with a nominal molecular cutoff a mass of 100 kDa (2), purified from fine impurities and, presumably, trimerized bsNT (3), delayed above the filter with a molecular weight cutoff of 100 kDa. The arrow shows the position of the monomeric bsNT. A marker (M) is applied in the rightmost track and the dimensions of the marker strips are indicated (in kDa). b – Immunoassay of binding of the obtained bsNT (HN-E7)x3, as well as control monovalent NATs (E7 – against IgA and HN13 – against hemagglutinin HA-H5 avian influenza virus) at a concentration of 1 mcg/ml with immobilized recombinant antigens (IgA and HA-H5). The control wells were without antigen (columns "without AG"). The averaged data of three dimensions are presented, indicating the area of data dispersion |