Molecular Species of Membrane Lipids of the Sea Anemone Exaiptasia diaphana and Its Symbionts

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Abstract

The molecular types of membrane lipids of the sea anemone Exaiptasia diaphana and the molecular types of glycolipids of its symbionts were studied using the method of high-performance liquid chromatography with mass spectrometric detection. A total of 82 molecular types of E. diaphana glycerophospholipids were identified, the main ones being 16:0/22:6 cholinoglycerophospholipid (PC), 18:1e/20:4 and 18:1e/20:5 ethanolaminoglycerophospholipids (PE), 18:0/22:4 serinoglycerophospholipid (PS), 18:0/22:4 inositoglycerophospholipid (PI), 18:2b/16:0 ceramidaminoethylphosphonate (CAEP). 36 molecular types of glycolipids have been identified in symbionts. The main molecular species were 18:4/18:5 monohalactosyldiacylglycerol (MGDG), 18:3/18:5 and 18:4/18:4 digalactosyldiacylglycerols DGDG, 14:0/16:0 sulfoquinovosyldiacylglycerol (SQDG). Molecular genetic analysis revealed that all E. diaphana colonies contained the following dinoflagellates: Breviolum minutum, Cladocopium thermophilum, and Gerakladium endoclionum. The profile of the molecular types of lipids of the host organism can act as a chemotaxonomic feature, and galactolipids of symbionts indicate resistance to changes in seawater temperature. This study contributes to the development of lipidomics of marine organisms of the phylum Cnidaria.

About the authors

E. T. Bizikashvili

A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch, Russian Academy of Sciences

Email: bilielena801@gmail.com
Russia, Vladivostok

S. A. Kozlovskiy

A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch, Russian Academy of Sciences

Russia, Vladivostok

E. V. Ermolenko

A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch, Russian Academy of Sciences

Russia, Vladivostok

K. V. Efimova

A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch, Russian Academy of Sciences

Russia, Vladivostok

T. V. Sikorskaya

A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch, Russian Academy of Sciences

Russia, Vladivostok

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