Fusarium species affecting potato tubers and tomato fruits in Uganda

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Resumo

Irish potato and tomato are among the most widely cultivated crops in Uganda. In 2020, samples of affected potato tubers and tomato fruits were collected from farms across four regions in Uganda for analysis. A total of 22 strains of Fusarium spp. were isolated from potato tubers and seven strains were isolated from tomato fruits. Identification of the fungal species was accomplished using cultural and morphological characteristics, as well as DNA sequencing targeting specific regions: ITS1–5.8S–ITS2, parts of the elongation factor 1 (tef 1) gene, and beta-tubulin (β-tub) gene. The analysis of the isolated strains from potato tubers revealed the presence of Fusarium incarnatum-equisety species complex, F. sambucinum species complex, F. oxysporum species complex, F. solani species complex. Additionally, F. incarnatum-equiseti species complex was detected in tomato fruits. All the investigated strains exhibited the ability to successfully infect both injured tomato fruits and potato tubers. Tested strains were susceptible to difenoconazole (ЕС50 = 0.08–8.5 mg/L) and thiabendazole (EC50 = 0.67–5.1 mg/L).

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Sobre autores

A. Elansky

Peoples Friendship University of Russia named after Patrice Lumumba

Autor responsável pela correspondência
Email: sasha.elansky@gmail.com
Rússia, 117198 Moscow

S. Mislavskiy

Peoples Friendship University of Russia named after Patrice Lumumba

Email: mislavskiy.sm@yandex.ru
Rússia, 117198 Moscow

E. Chudinova

Peoples Friendship University of Russia named after Patrice Lumumba

Email: chudiel@mail.ru
Rússia, 117198 Moscow

L. Kokaeva

Peoples Friendship University of Russia named after Patrice Lumumba; M.V. Lomonosov Moscow State University

Email: kokaeval@gmail.com
Rússia, 117198 Moscow; 119991 Moscow

S. Elansky

Peoples Friendship University of Russia named after Patrice Lumumba; M.V. Lomonosov Moscow State University

Email: elanskiy_sn@pfur.ru
Rússia, 117198 Moscow; 119991 Moscow

E. Denisova

M.V. Lomonosov Moscow State University

Email: denisova.elizavet@gmail.com
Rússia, 119991 Moscow

I. Ilichev

M.V. Lomonosov Moscow State University

Email: igor.ilichev.msu@gmail.com
Rússia, 119991 Moscow

A. Belosokhov

M.V. Lomonosov Moscow State University

Email: arsenybelosokhov.msu.bios@gmail.com
Rússia, 119991 Moscow

Yu. Bamutaze

Makerere University

Email: yazidhibamutaze@gmail.com

Department of Geography, Geo-Informatics and Climatic Sciences

Uganda, 7062 Kampala

P. Musinguzi

Makerere University

Email: patrick.musinguzi@mak.ac.ug

Department of Agricultural Production, School of Agricultural Sciences

Uganda, 7062 Kampala

E. Opolot

Makerere University

Email: oplote@yahoo.com

Department of Agricultural Production, School of Agricultural Sciences

Uganda, 7062 Kampala

P. Krasilnikov

M.V. Lomonosov Moscow State University

Email: pavel.krasilnikov@gmail.com
Rússia, 119991 Moscow

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2. Fig. 1. Samples of infected potato tubers and tomato fruits were collected samples were collected in the southwest (1, 2), center (3) and east (4) of Uganda.

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3. Fig. 2. Phylogenetic tree inferred from maximum-likelihood analysis of the concatenated alignment, including partial tef gene region (675 bp). The confidence values are indicated at the branches (1000 bootstrap replicates). PT, PL, PS — isolates from potato tubers, leaves, stems correspondingly, TF — from tomato fruits, SC — species complex.

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4. Fig. 3. Phylogenetic tree inferred from maximum-likelihood analysis of the concatenated alignment, including partial tef and tub gene regions (1230 bp). The confidence values are indicated at the branches (500 bootstrap replicates). PT, PL, PS — isolates from potato tubers, leaves, stems correspondingly, TF — from tomato fruits.

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