The study of the morphological and anatomical structure of the Tuberosus chin

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Abstract

Introduction. Tuberosus chin (Lathyrus tuberosus L.) is a plant widely represented in the chernozem zone of Russia. It contains various groups of biologically active substances: flavonoids, polysaccharides, terpenoids, tannins, amino acids. The plant is used in folk medicine. There are no data on the morphological and microscopic examination of the raw materials of the Lathyrus tuberosus.

Objective: establishing indicators of the authenticity of the of Lathyrus tuberosus herb, including studies of morphological and microdiagnostic characteristics of raw materials.

Material and methods. As an object of study was used the aerial part of the Lathyrus tuberosus, both fresh and dried, collected in the Kursk region in 2019-2021. Macroscopic studies were carried out with the naked eye and using a binocular loupe. Microscopic signs of various organs of the Lathyrus tuberosus were analyzed by preparing temporary preparations, using a «Micromed C1 LED» microscope with a digital attachment.

Results. Morphological and microdiagnostic features of Lathyrus tuberosus herb, including stem, leaf, calyx and corolla, have been established. The main microdiagnostic features are: the pedicle is of a bundle type of structure, the vascular bundles are closed, collateral. Anomocytic stomata. The pubescence includes three types of hairs: three-celled with thick-walled cells, their basal part consists of two small prosenchymal cells, the terminal cell is long with a pointed apex (leaflets of a compound leaf); simple thin-walled one-two-celled hairs with a pointed apex and simple thin-walled two-three-celled hairs with an elongated terminal cell and a rounded apex with granular contents (calyx). The edge of the corolla has thin-walled papillary outgrowths. Prismatic crystals are located along the veins of the leaflets of the compound leaf and the calyx.

Conclusion. The investigation has provided new data on the morphological and anatomical structure of Lathyrus tuberosus herb.

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About the authors

Ksenia Romanovna Bubenchikova

Kursk State Medical University

Author for correspondence.
Email: bubenhikova.ksmu@yandex.ru

3th year student

Irina Vladimirovna Paskar

LLC EC "PHARMOBORONA"

Email: paskar_irina@farmoborona.ru
Candidate of Pharmaceutical Sciences, General Director

References

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2. Fig. 1. Microscopy of the stem and leaf of the Lathyrus tuberosus: a – cross section of the stem, ×60; b – a fragment of a transverse section, ×150; c – a fragment of the stem epidermis with stomata, ×150; d – fragment of the lower epidermis of leaflets with stomata, ×150; e – a fragment of the upper epidermis of leaflets with stomata, ×150; f – a fragment of the epidermis with leaf veins, a simple thick-walled hair with a smooth cuticle, ×150; g – a fragment of the epidermis of the lower part of the leaflet, a three-celled thick-walled hair with a smooth cuticle, ×150; h – a fragment of the edge of a leaflet, ×120; i – a fragment of the epidermis of the lower part of the leaflet, ×300

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3. Fig. 2. Microscopy of the calyx and corolla of the Lathyrus tuberosus: a – a fragment of the epidermis in the cup tube, ×150; b – a fragment of the epidermis in the throat of the calyx with stomata, ×150; c – a fragment of the calyx margin and simple thin-walled hairs, ×150; d – a fragment of the epidermis of the calyx and simple thin-walled hairs with granular content, ×150; e – a fragment of the epidermis at the base of the corolla, ×150; f – a fragment of the epidermis in the middle part of the corolla, ×150; g – a fragment of the epidermis of the corolla on the fold from the inside, the side of ×300; h – a fragment of the epidermis on the fold from the outside and epidermal cells with papillary outgrowths, ×300; i – a fragment of the corolla edge with simple papillary hairs, ×150

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